PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation
نویسندگان
چکیده
1. High assay specificity without use of a probe. Despite the fact that probes may increase the detection specificity of an assay, undetected coamplification of nonspecific products (off-target amplification and primerdimers) may negatively impact the performance of the assay, even for probe-based assays. PrimePCR assays are based on state-of-the-art specificity prediction algorithms, followed by next generation sequence verification of each amplicon.
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تاریخ انتشار 2012